Human B2-glycoprotein 1 (beta2-GP1) antibody lgG ELISA Kit

Artikelnummer: BYT-ORB1496507
Artikelname: Human B2-glycoprotein 1 (beta2-GP1) antibody lgG ELISA Kit
Artikelnummer: BYT-ORB1496507
Hersteller Artikelnummer: orb1496507
Alternativnummer: BYT-ORB1496507-48,BYT-ORB1496507-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Spezies Reaktivität: Human
Alternative Synonym: APOH, BG, B2G1, B2GP1
Human B2-glycoprotein 1 antibody lgG ELISA kit
Konzentration: 10 ng/mL
Detektionsbereich: 0.16-10 ng/mL
Sensitivitaet: 0.06 ng/mL
Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung: Application Notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human beta2-GP1 lgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human beta2-GP1 lgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human beta2-GP1 lgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human beta2-GP1 lgG in the samples is then determined by comparing the OD of the samples to the standard curve
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