Neuroligin 1 Antibody (RPE), IgG1, Clone: [N97A/31 Formerly sold as S97A-31], Mouse, Monoclonal

Produktübersicht

• Artikelname: Neuroligin 1 Antibody (RPE), IgG1, Clone: [N97A/31 Formerly sold as S97A-31], Mouse, Monoclonal
• Artikelnummer: BYT-ORB150890
• Hersteller Artikelnummer: orb150890
• Alternativnummer: BYT-ORB150890-100
• Hersteller: Biorbyt
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ICC, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Fusion protein amino acids 718-843 (cytoplasmic C-terminus) of rat Neuroligin-1. Mouse: 99% identity (125/126 amino acids identical). Human: 99% identity (125/126 amino acids identical) > 40% identity with Neuroligin-2 and -3.
• Konjugation: RPE
• Alternative Synonym: NLG 1, KIAA1070, MGC45115, Neuroligin-1, NL1, NLG1, Nlgn1

Produktbeschreibung

Mouse monoclonal to Neuroligin 1 (RPE). Neuroligin-1 is a neuronal cell surface protein belonging to the type-B carboxylesterase/lipase family. It is a necessary component in the maturation of excitatory synapses for their normal, functional development, and is necessary to the regulation of synaptic plasticity and the development of long-term memory within the adult amygdala in mammals. It is believed to participate in cell-cell-interaction through the assembly of intracellular junction by the binding of beta-neurexins, and may also be a factor in the maintenance and assembly of synaptic junctions. It is also thought to have involvement in excitatory synaptic specification. Within brain tissue, Neuroligin-1 is primarily observed in neurons and spinal cord..

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1 mg/ml
• Klon-Bezeichnung: [N97A/31 Formerly sold as S97A-31]
• Molekulargewicht: 120kDa
• Isotyp: IgG1
• NCBI: 446320
• UniProt: Q62765
• Puffer: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Target-Kategorie: Neuroligin 1

Anwendungsbeschreibung

• Application Verdünnung: WB (1:1000), ICC/IF (1:100)
• Anwendungsbeschreibung: Application Notes: 2 µg/ml of SMC-463 was sufficient for detection of Neuroligin-1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody

Bilder

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 1 Monoclonal Antibody, Clone N97A/31. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Neuroligin 1 Monoclonal Antibody at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain, Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Neuroligin 1 Antibody (D) Composite.