Rabbit anti-SMC1 Recombinant Monoclonal Antibody, Clone: [BL-205-2G8], Unconjugated

Produktübersicht

• Artikelname: Rabbit anti-SMC1 Recombinant Monoclonal Antibody, Clone: [BL-205-2G8], Unconjugated
• Artikelnummer: BYT-ORB1519958
• Hersteller Artikelnummer: orb1519958
• Alternativnummer: BYT-ORB1519958-100
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, ICC, IHC, IP, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Between 1183 and C-terminus
• Konjugation: Unconjugated
• Alternative Synonym: SMC protein 1A, SMC-1A, structural maintenance of chromosomes protein 1A, SMCB, SMC1L1, SMC-1-alpha, SMC1alpha, SMC1, segregation of mitotic chromosomes 1, SB1.8, epididymis secretory sperm binding protein, DXS423E, CDLS2, SMC1 (structural maintenance of chromosomes 1, yeast)-like 1

Produktbeschreibung

SMC1 Antibody

Produkteigenschaften

• Klonalität: Recombinant
• Konzentration: 50 µg/ml
• Klon-Bezeichnung: [BL-205-2G8]
• NCBI: 006297
• UniProt: Q14683
• Puffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Formulierung: Whole IgG
• Target-Kategorie: SMC1

Anwendungsbeschreibung

• Application Verdünnung: WB - 1:1000, IP - 20 µl/mg lysate, IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections., IHC-IF
• Anwendungsbeschreibung: Application Notes: Format: Whole IgG

Bilder

Detection of mouse SMC1 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (orb1519958) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Detection of human SMC1 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (orb1519958) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Detection of human SMC1 by immunocytochemistry. Sample: FFPE section of NCI-H226 cells. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human SMC1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse SMC1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human SMC1 in FFPE breast carcinoma by immunohistochemistry-IF. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal(TM). Counterstain: DAPI.

Detection of human SMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958) used for IP at 20 µl/mg lysate.

Detection of mouse SMC1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Detection of human SMC1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, MCF-7, Jurkat, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.