Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Formulierung:
Whole IgG
Target-Kategorie:
PAF
Application Verdünnung:
WB - 1:2,000 - 1:10,000, IP - Not recommended, IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Anwendungsbeschreibung:
Application Notes: Format: Whole IgG
Detection of human PAF by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-PAF (Cat. No. orb1523112) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Detection of human PAF by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN or RIPA buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PAF antibody orb1523112 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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