Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Formulierung:
Whole IgG
Target-Kategorie:
PAF
Application Verdünnung:
WB - Not recommended. Use rabbit anti-PAF antibody A304-374A., IP - 2 - 10 µg/mg lysate, IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Anwendungsbeschreibung:
Application Notes: Format: Whole IgG
Detection of human PAF by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-PAF (Cat. No. orb1523114) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Detection of human PAF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction, 20% of IP loaded) prepared using RIPA buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-PAF antibody orb1523114 used for IP at 6 µg per reaction.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten