Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Formulierung:
Whole IgG
Target-Kategorie:
NOPP140
Application Verdünnung:
WB - 1:2,000 - 1:10,000, IP - 5 - 15 µg/mg lysate
Anwendungsbeschreibung:
Application Notes: Format: Whole IgG
Detection of human NOPP140 by western blot and immunoprecipitation. Samples: Whole cell lysate from K-562 (5, 15 and 50 µg for WB, 1 mg for IP, 20% of IP loaded) and HeLa (H, 50 µg) cells. Antibodies: Affinity purified rabbit anti-NOPP140 antibody orb1527062 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. NOPP140 was also immunoprecipitated by rabbit anti-NOPP140 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
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