Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Formulierung:
Whole IgG
Target-Kategorie:
NOPP140
Application Verdünnung:
WB - Not recommended. Use rabbit anti-NOPP140 antibody A302-185A., IP - 5 - 15 µg/mg lysate, IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Anwendungsbeschreibung:
Application Notes: Format: Whole IgG
Detection of human NOPP140 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-NOPP140 (Cat. No. orb1527063) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Detection of human NOPP140 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from K-562 cells. Antibodies: Affinity purified rabbit anti-NOPP140 antibody orb1527063 used for IP at 3 µg/mg lysate.
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