Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
Formulierung:
Whole IgG
Target-Kategorie:
TIF1 Alpha/TRIM24
Application Verdünnung:
WB - 1:2,000 - 1:10,000, IP - 2 - 10 µg/mg lysate, IHC - 1:200 - 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Anwendungsbeschreibung:
Application Notes: Format: Whole IgG
Localization of TIF1 Alpha/TRIM24 Binding Sites by ChIP-sequencing.Chromatin from Breast cancer cell line T47D was immunoprecipitated with anti-TIF1 Alpha/TRIM24 antibody and analyzed by DNA sequencing.
Detection of mouse TIF1 alpha/TRIM24 by immunohistochemistry. Sample: FFPE section of mouse intestine. Antibody: Affinity purified rabbit anti-TIF1 alpha/TRIM24 used at a dilution of 1:200 (1µg/ml).
Detection of human TIF1 alpha/TRIM24 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-TIF1 alpha/TRIM24 used at a dilution of 1:200 (1µg/ml).
Detection of human TIF1A by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-TIF1A antibody.
Detection of mouse TIF1A by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-TIF1A antibody at 0.1 µg/ml.
Detection of human TIF1A by western blot.Samples: Whole cell lysate from K-562, HEK293T, HeLa, MCF-7, and Hep-G2 cells. Antibody: Affinity purified rabbit anti-TIF1A antibody at 0.1 µg/ml.
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