Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot: (1:100) vs. full-length recombinant human ACE2 protein. Lane 7: 50 ng, Lane 8: 100 ng, Lane 9 500ng. Detection fluorescent conjugated anti-rabbit secondary antibody (1:100).
Indirect detection ELISA against full length Recombinant Human ACE2. Primary antibody orb1533473 was diluted 1:100 (10 ug/ml final concentration). Secondary goat anti-rabbit HRP conjugated antibody was diluted 1:100.
Capillary Western Analysis of anti-ACE2 antibody (orb1533473, 20 µg/mL) using 12-230 kDa separation module.
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