Anti-Glypican-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human glypican-1 protein.
Further information: This protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~61 kD in size corresponding to glypican by western blotting in the appropriate cell lysate or extract. The higher molecular weight (110kD) of transfected Fc-glypican compared with the expected MW of glypican is likely due to the presence of the Fc-tag.
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