ADORA2A/Adenosine A2A Receptor Antibody, IgG2a, Clone: [7F6-G5-A2 / 7F6], Unconjugated, Mouse, Monoclonal

Artikelnummer: BYT-ORB1537749
Artikelname: ADORA2A/Adenosine A2A Receptor Antibody, IgG2a, Clone: [7F6-G5-A2 / 7F6], Unconjugated, Mouse, Monoclonal
Artikelnummer: BYT-ORB1537749
Hersteller Artikelnummer: orb1537749
Alternativnummer: BYT-ORB1537749-50
Hersteller: Biorbyt
Wirt: Mouse
Kategorie: Antikörper
Applikation: FC, IHC, IHC-P, WB
Spezies Reaktivität: Canine, Guinea pig, Human, Mouse, Rabbit, Rat
Immunogen: 6His-tagged full-length human recombinant A2A receptor. Epitope mapped to SQPLPGER in the third intracellular loop.
Konjugation: Unconjugated
Alternative Synonym: ADORA2A, Adenosine receptor A2a, ADORA2, A2a receptor, Adenosine A2a receptor, Adenosine A2 receptor, Adenosine receptor subtype A2a, HA2aR, RDC8, AA2AR, A2AR
ADORA2A/Adenosine A2A Receptor Antibody
Klonalität: Monoclonal
Konzentration: 0.5 mg/ml
Klon-Bezeichnung: [7F6-G5-A2 / 7F6]
Isotyp: IgG2a
Puffer: 0.01M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide
Target-Kategorie: ADORA2A/Adenosine A2A Receptor
Application Verdünnung: Flo, IHC, IHC-P (5 µg/ml), IHC-Pfa (1 µg/ml), WB (1:1000)
Anwendungsbeschreibung: Application Notes: Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 5 ug/ml. Western Blot: 1:1000-1:5000 detected Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras