DC-SIGN Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: DC-SIGN Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB156554
• Hersteller Artikelnummer: orb156554
• Alternativnummer: BYT-ORB156554-100,BYT-ORB156554-200,BYT-ORB156554-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human
• Immunogen: KLH conjugated synthetic peptide derived from human DC-SIGN/CD209 (51-150/1404aa)
• Konjugation: Unconjugated
• Alternative Synonym: CLEC4L, Dendritic cell-specific ICAM-3-grabbing non-integrin 1, C type lectin domain family 4 member L, CD 209, CD209, CD209 antigen, CD209 antigen-like protein A, CD209 molecule, Cd209a, CDSIGN, CIRE, DC SIGN1, DCSIGN, Dendritic cell specific ICAM 3 grabbing nonintegrin 1, Dendritic cell specific ICAM3 grabbing nonintegrin 1, Dendritic cell-specific intracellular adhesion molecules(ICAM)-3 grabbing non-integrin, Dengue fever, protection against, included, Dentritic Cell Specific ICAM3 Grabbing Nonintegrin, HIV GP120 Binding Protein, MGC129965, MGC130443, SIGN-R1, SIGNR5, CD209_HUMAN.

Produktbeschreibung

DC-SIGN Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 45 kDa
• UniProt: Q9NNX6
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: CD209

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test

Bilder

Flow cytometric analysis of mouse spleen cell using DC-SIGN antibody.

Blank control (blue line): MCF7 (blue). Primary Antibody (green line): Rabbit Anti-DC-SIGN antibody, Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): F (ab)2 fragment goat anti-rabbit IgG-FITC. Dilution: 1 µg/Test. Protocol, The cells were fixed with 2% paraformaldehyde for 10 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DC-SIGN) Polyclonal Antibody, Unconjugated (orb156554) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (DC-SIGN) Polyclonal Antibody, Unconjugated (orb156554) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Hela Cell Lysate at 40 ug, Hcclm3 Cell Lysate at 40 ug, Primary: Anti-DC-SIGN (orb156554) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 50 kD.