Phospho-HSP70 (Tyr611) Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: Phospho-HSP70 (Tyr611) Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB157590
• Hersteller Artikelnummer: orb157590
• Alternativnummer: BYT-ORB157590-100,BYT-ORB157590-200,BYT-ORB157590-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, IF, IHC-Fr, IHC-P
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human HSP70 around the phosphorylation site of Tyr611 GL(p-Y)QG
• Konjugation: Unconjugated
• Alternative Synonym: HSP70(phospho Tyr611), HSP70(phospho Y611), p-HSP70(Tyr611), p-HSP70(Y611), HSP70, HSP-70, HSP 70, Heat shock 70 kDa protein 1, heat shock 70kDa protein 1A, Heat shock 70kDa protein 1B, Heat shock induced protein, heat shock protein 70, HSP70 1, HSP70 2, HSP70.1, HSP72, HSPA1, HSPA1A, HSPA1B, XXbac BCX40G17.3 001, Heat shock 70 kDa protein 1A/1B, HSP71A_HUMAN, Hspa1a, Hsp70-1, HSP72, Hspa1, Hspa1b, Heat shock 70 kDa protein 1A/1B, shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP70.1/HSP70.2.

Produktbeschreibung

Phospho-HSP70 (Tyr611) Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 70 kDa
• UniProt: P0DMV8
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: HSPA1A

Anwendungsbeschreibung

• Application Verdünnung: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
• Anwendungsbeschreibung: Modification: Phosphorylated

Bilder

Flow cytometric analysis of Jurkat cells using HSP70 (Phospho-Tyr611) antibody.

Blank control (blue line): Jurkat (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-phospho-HSP70 (Tyr611) antibody (orb157590), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/Test.

Blank control: A549. Primary Antibody (green line): Rabbit Anti-phospho-HSP70 (Tyr611) antibody (orb157590), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-HSP70 (Tyr611)) Polyclonal Antibody, Unconjugated (orb157590) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human endometrial carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-HSP70 (Tyr611)) Polyclonal Antibody, Unconjugated (orb157590) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-HSP70 (Tyr611)) Polyclonal Antibody, Unconjugated (orb157590) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human liver cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-HSP70 (Tyr611)) Polyclonal Antibody, Unconjugated (orb157590) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-HSP70 (Tyr611)) Polyclonal Antibody, Unconjugated (orb157590) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-HSP70 (Tyr611)) Polyclonal Antibody, Unconjugated (orb157590) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.