Produced from sera of rabbits immunized with highly pure Recombinant Human Amphiregulin. Anti-Human Amphiregulin-specific antibody was purified by affinity chromatography and then biotinylated.
Application Notes: Sandwich ELISA: To detect Human Amphiregulin by sandwich ELISA (using 100µl/well), a concentration of 0.25-1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyts Polyclonal Anti-Human Amphiregulin as a capture antibody, allows the detection of at least 2000-4000 pg/ml ofRecombinant Human Amphiregulin. Western Blot To detect Human Amphiregulin by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human Amphiregulin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
To detect Human Amphiregulin by sandwich ELISA (using 100 µl/well), a concentration of 0.25-1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Amphiregulin as a capture antibody, allows the detection of at least 2000-4000 pg/ml ofRecombinant Human Amphiregulin.
To detect Human Amphiregulin by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human Amphiregulin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human Amphiregulin by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human Amphiregulin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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