S100A10 Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: S100A10 Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB1704
• Hersteller Artikelnummer: orb1704
• Alternativnummer: BYT-ORB1704-100,BYT-ORB1704-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human S100A10 (21-97/97aa)
• Konjugation: Unconjugated
• Alternative Synonym: Alternative names42C, Annexin II ligand, Annexin II ligand, calpactin I, light polypeptide, Annexin II, light chain, ANX2L, ANX2LG, CAL12, CAL1L, Calpactin I light chain, Calpactin I, p11 subunit, Calpactin-1 light chain, Cellular ligand of annexin II, CLP11, GP11, Nerve growth factor-induced protein 42C, p10 antibody p10 protein, p11, Protein S100 A10, Protein S100-A10, S100 calcium binding protein A10(annexin II ligand, calpactin I, light polypeptide(p11)), S100 calcium binding protein A10(calpactin), S100 calcium binding protein A10, S100 calcium-binding protein A10, S100a10, S10AA_HUMAN.

Produktbeschreibung

S100A10 Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 11 kDa
• UniProt: P60903
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: S100A10

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200, ELISA=1:5000-10000

Bilder

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (S100A10) Polyclonal Antibody, Unconjugated (orb1704) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (S100A10) Polyclonal Antibody, Unconjugated (orb1704) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (S100A10) Polyclonal Antibody, Unconjugated (orb1704) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (S100A10) Polyclonal Antibody, Unconjugated (orb1704) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (S100A10) Polyclonal Antibody, Unconjugated (orb1704) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (S100A10) Polyclonal Antibody, Unconjugated (orb1704) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (S100A10) Polyclonal Antibody, Unconjugated (orb1704) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Heart tissue lysates, Lane 2: Mouse Heart tissue lysates, Primary: Anti-S100A10 (orb1704) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 11 kDa, Observed band size: 13 kDa.

Tissue/Cell: Human laryngeal tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-S100A10 Polyclonal Antibody, Unconjugated (orb1704) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.