Human IL-35 R, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Glu 619 & Lys 24 - Asn 622 (Accession P40189-1(gp130) & Q99665-1(IL12Rb2)).
Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 73.1 kDa & 71.5 kDa. The protein migrates as 95-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation
Immobilized Human IL-35 R, His Tag (Cat. No. orb1710809) at 5 µg/mL (100 µL/well) can bind Human IL-35, Fc Tag (Cat. No. orb1184774) with a linear range of 0.002-0.625 µg/mL (QC tested).
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