Rabbit anti-LYRIC/AEG1 Recombinant Monoclonal Antibody, Clone: [BLR258L], Unconjugated

Produktübersicht

• Artikelname: Rabbit anti-LYRIC/AEG1 Recombinant Monoclonal Antibody, Clone: [BLR258L], Unconjugated
• Artikelnummer: BYT-ORB1710822
• Hersteller Artikelnummer: orb1710822
• Alternativnummer: BYT-ORB1710822-10
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, ICC, IHC, IP, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Between 475 and 525
• Konjugation: Unconjugated
• Alternative Synonym: astrocyte elevated gene-1 protein, protein LYRIC, metastasis adhesion protein, Metadherin, lysine-rich CEACAM1 co-isolated protein, LYRIC, AEG-1, AEG1, 3D3/LYRIC, 3D3, astrocyte elevated gene 1, LYRIC/3D3

Produktbeschreibung

LYRIC/AEG1 Antibody

Produkteigenschaften

• Klonalität: Recombinant
• Konzentration: 250 µg/ml
• Klon-Bezeichnung: [BLR258L]
• UniProt: Q86UE4
• Puffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Formulierung: Whole IgG
• Target-Kategorie: LYRIC/AEG1

Anwendungsbeschreibung

• Application Verdünnung: WB: 1:1,000, IP: 20 µl/1 mg lysate, IHC: 1:50 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC: 1:50 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Anwendungsbeschreibung: Application Notes: Format: Whole IgG

Bilder

Detection of human LYRIC/AEG1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (orb1710822) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Detection of mouse LYRIC/AEG1 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (orb1710822) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Detection of human LYRIC/AEG1 by immunocytochemistry. Sample: FFPE section of human RKO cells. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (orb1710822). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse LYRIC/AEG1 by immunocytochemistry. Sample: FFPE section of mouse CT26 cells. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (orb1710822). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human LYRIC/AEG1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (orb1710822). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human LYRIC/AEG1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (orb1710822). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human LYRIC/AEG1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 20% of IP loaded) from RKO cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (orb1710822) used for IP at 20 µl/mg lysate.

Detection of mouse LYRIC/AEG1 by western blot. Samples: Whole cell lysate (25 µg) from NIH 3T3, ND7/23, CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (orb1710822) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human LYRIC/AEG1 by western blot. Samples: Whole cell lysate (10 µg) from RKO, LNCaP, A-549, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (orb1710822) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.