WB: 1:1,000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Anwendungsbeschreibung:
Application Notes: Format: Whole IgG
Detection of mouse JMJD6 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
Detection of human JMJD6 (shaded) in A549 cells by flow cytometry. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
Detection of human JMJD6 by immunocytochemistry. Sample: FFPE section of human A549 cells. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JMJD6 by immunocytochemistry. Sample: FFPE section of mouse ND7/23 cells. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human JMJD6 by immunohistochemistry. Sample: FFPE section of human testis. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JMJD6 by immunohistochemistry. Sample: FFPE section of mouse testis. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JMJD6 by western blot. Samples: Whole cell lysate (10 µg) from BW5147.3, NIH 3T3, CH27, ND7/23, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human JMJD6 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Hep-G2, RKO, and OVCAR-8 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
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