Rabbit anti-JMJD6 Recombinant Monoclonal Antibody, Clone: [BLR254L], Unconjugated

Artikelnummer: BYT-ORB1710830
Artikelname: Rabbit anti-JMJD6 Recombinant Monoclonal Antibody, Clone: [BLR254L], Unconjugated
Artikelnummer: BYT-ORB1710830
Hersteller Artikelnummer: orb1710830
Alternativnummer: BYT-ORB1710830-10
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: FC, ICC, IHC, WB
Spezies Reaktivität: Human, Mouse
Immunogen: Between 125 and 175
Konjugation: Unconjugated
Alternative Synonym: peptide-lysine 5-dioxygenase JMJD6, PTDSR1, PTDSR, PSR, phosphatidylserine receptor, lysyl-hydroxylase JMJD6, jumonji domain-containing protein 6, jumonji domain containing 6, jmjC domain-containing protein 6, histone arginine demethylase JMJD6, bifunctional arginine demethylase and lysyl-hydroxylase JMJD6, arginine demethylase and lysine hydroxylase, Protein PTDSR
JMJD6 Antibody
Klonalität: Recombinant
Konzentration: 1000 µg/ml
Klon-Bezeichnung: [BLR254L]
UniProt: Q6NYC1
Puffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Formulierung: Whole IgG
Target-Kategorie: JMJD6
Application Verdünnung: WB: 1:1,000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Anwendungsbeschreibung: Application Notes: Format: Whole IgG
Detection of mouse JMJD6 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
Detection of human JMJD6 (shaded) in A549 cells by flow cytometry. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
Detection of human JMJD6 by immunocytochemistry. Sample: FFPE section of human A549 cells. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JMJD6 by immunocytochemistry. Sample: FFPE section of mouse ND7/23 cells. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human JMJD6 by immunohistochemistry. Sample: FFPE section of human testis. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JMJD6 by immunohistochemistry. Sample: FFPE section of mouse testis. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JMJD6 by western blot. Samples: Whole cell lysate (10 µg) from BW5147.3, NIH 3T3, CH27, ND7/23, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human JMJD6 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Hep-G2, RKO, and OVCAR-8 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.