Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Formulierung:
Whole IgG
Target-Kategorie:
JDP2
Application Verdünnung:
WB: 1:1000, IHC: 1:40 to 1:100. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections, ICC: 1:40 to 1:100. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE cell sections
Anwendungsbeschreibung:
Application Notes: Format: Whole IgG
Detection of human JDP2 (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733243) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
Detection of mouse JDP2 (shaded) in CTLL-2 cells by flow cytometry. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733243) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
Detection of mouse JDP2 by immunocytochemistry. Sample: FFPE section of mouse C2C12 cells. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733243). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human JDP2 by immunocytochemistry. Sample: FFPE section of human FaDu cells. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733243). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human JDP2 by immunohistochemistry. Sample: FFPE section of human spleen. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733243). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human JDP2 by immunohistochemistry. Sample: FFPE section of human skin carcinoma. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733243). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JDP2 by immunohistochemistry. Sample: FFPE section of mouse RenCa tumor. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733243). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse JDP2 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 and RenCa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733243) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human JDP2 by western blot. Samples: Whole cell lysate (25 µg) from MCF-7, HeLa, HaCaT, and DU145 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733243) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
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