Rabbit anti-KLF5 Recombinant Monoclonal Antibody, Clone: [BLR243L], Unconjugated

Produktübersicht

• Artikelname: Rabbit anti-KLF5 Recombinant Monoclonal Antibody, Clone: [BLR243L], Unconjugated
• Artikelnummer: BYT-ORB1733252
• Hersteller Artikelnummer: orb1733252
• Alternativnummer: BYT-ORB1733252-10
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, ICC, IHC, IP, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Between 325 and 375
• Konjugation: Unconjugated
• Alternative Synonym: GC-box-binding protein 2, Kruppel-like factor 5 (intestinal), Krueppel-like factor 5, Klf5C isoform, intestinal-enriched kruppel-like factor, intestinal-enriched krueppel-like factor, intestinal Kruppel-like factor, IKLF, epididymis secretory sperm binding protein, colon kruppel-like factor, colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2, basic transcription element binding protein 2, transcription factor BTEB2, GC box binding protein 2

Produktbeschreibung

KLF5 Antibody

Produkteigenschaften

• Klonalität: Recombinant
• Konzentration: 1000 µg/ml
• Klon-Bezeichnung: [BLR243L]
• UniProt: Q13887
• Puffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Formulierung: Whole IgG
• Target-Kategorie: KLF5

Anwendungsbeschreibung

• Application Verdünnung: WB: 1:1000, IP: 6 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Anwendungsbeschreibung: Application Notes: Format: Whole IgG

Bilder

Detection of human KLF5 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Detection of mouse KLF5 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Detection of human KLF5 by immunocytochemistry. Sample: FFPE section of HT-29 cells. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse KLF5 by immunocytochemistry. Sample: FFPE section of CT26 cells. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse KLF5 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human KLF5 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human KLF5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 20% of IP loaded) from KM12 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252) used for IP at 6 µl/mg lysate.

Detection of mouse KLF5 by western blot. Samples: Whole cell lysate (25 µg) from CT26, WEHI-231, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human KLF5 by western blot. Samples: Whole cell lysate (25 µg) from HeLa, SW620, MCF-7, and KM12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.