5-Vinyl-2-deoxyuridine (5-VdU), CAS [[55520-67-7]]
Artikelnummer:
BYT-ORB1734969
Hersteller Artikelnummer:
orb1734969
Alternativnummer:
BYT-ORB1734969-50
Hersteller:
Biorbyt
Kategorie:
Biochemikalien
Alternative Synonym:
5-Ethenyl-2-deoxyuridine, 2-Deoxy-5-vinyluridine
5-VdU (5-Vinyl-2-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2-deoxyuridine) or the copper-catalyst requiring 5-EdU (5-Ethynyl-2-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-VdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymidine. The resulting vinyl-functionalized DNA can subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging.
