Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization
Target-Kategorie:
CCR8
Elisa plates were pre-coated with Flag Tag CCR8-Nanodisc (0.2 µg/per well). Serial diluted anti-CCR8 monoclonal antibody (orb1291212) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CCR8 monoclonal antibody binding with CCR8-Nanodisc is 12.07 ng/ml.
WB analysis of Human CCR8-Nanodisc with anti-CCR8 monoclonal antibody (orb1291212) at 1 µg/ml, followed by Goat Anti-Human IgG HRP at 1/5000 dilution
Human CCR8-Nanodisc can bind Anti-CCR8 antibody (orb1291212) with an affinity constant of 1.408 nM as determined in a SPR assay.
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