HOXD10 Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: HOXD10 Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: BYT-ORB1743885
• Hersteller Artikelnummer: orb1743885
• Alternativnummer: BYT-ORB1743885-100
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IHC, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: E.coli-derived human HOXD10 recombinant protein (Position: A9-K252).
• Konjugation: Unconjugated
• Alternative Synonym: Transcription factor MafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3b1 factor, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, MAFA

Produktbeschreibung

Anti-HOXD10 Antibody. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
• Molekulargewicht: 40 kDa
• UniProt: P28358
• Formulierung: Lyophilized
• Target-Kategorie: Zinc finger protein Helios

Anwendungsbeschreibung

• Anwendungsbeschreibung: Application Notes: Western blot, 0.25-0.5 µg/ml, Mouse Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human ELISA, 0.1-0.5 µg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml

Bilder

WB analysis of HOXD10 using anti-HOXD10 antibody.Lane 1:mouse heart tissue.

IHC analysis of HOXD10 using anti-HOXD10 antibody. HOXD10 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-HOXD10 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of HOXD10 using anti-HOXD10 antibody. HOXD10 was detected in a paraffin-embedded section of human mucinous adenoma of ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-HOXD10 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of HOXD10 using anti-HOXD10 antibody. HOXD10 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-HOXD10 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of HOXD10 using anti-HOXD10 antibody. HOXD10 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-HOXD10 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of HOXD10 using anti-HOXD10 antibody. HOXD10 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-HOXD10 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of HOXD10 using anti-HOXD10 antibody. HOXD10 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-HOXD10 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Western blot analysis of HOXD10 using anti-HOXD10 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates. After electrophoresi