Detection of human PVRL1/NECTIN1 (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784566) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
Detection of human PVRL1/NECTIN1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 20% of IP loaded) from MCF-10A cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784566) used for IP at 6 µl/mg lysate.
Detection of human PVRL1/NECTIN1 by western blot. Samples: Whole cell lysate (50 µg) from MCF-10A, SNB-75, RT-4, MCF-7, and HaCaT cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784566) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten