Rabbit PVRL1/NECTIN1 Recombinant Monoclonal Antibody, Clone: [BLR274L], Unconjugated

Artikelnummer: BYT-ORB1784567
Artikelname: Rabbit PVRL1/NECTIN1 Recombinant Monoclonal Antibody, Clone: [BLR274L], Unconjugated
Artikelnummer: BYT-ORB1784567
Hersteller Artikelnummer: orb1784567
Alternativnummer: BYT-ORB1784567-100,BYT-ORB1784567-20
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: FC, IP, WB
Spezies Reaktivität: Human
Immunogen: Residues 31-331 (ECD)
Konjugation: Unconjugated
Alternative Synonym: CD antigen CD111,CD111,CLPED1,ectodermal dysplasia 4 (Margarita Island type),ED4,herpes simplex virus type 1 sensitivity,herpes virus entry mediator C,Herpesvirus entry mediator C,herpesvirus Ig-like receptor,HIgR,HV1S,HVEC,nectin 1,Nectin cell adhesion molecule 1,nectin-1,OFC7,poliovirus receptor-like 1,poliovirus receptor-related 1 (herpesvirus entry mediator C),poliovirus receptor-related protein 1,PRR,PRR1,PVRL1,PVRR,PVRR1,SK-12
Rabbit PVRL1/NECTIN1 Recombinant Monoclonal Antibody
Klonalität: Recombinant
Konzentration: 1000 µg/ml
Klon-Bezeichnung: [BLR274L]
NCBI: 002846
UniProt: Q15223
Puffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
Formulierung: Liquid
Target-Kategorie: PVRL1/NECTIN1
Application Verdünnung: Application : Flow-Cyt , ApplicationDilutionRange : Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10 6 cells. , Application : IP , ApplicationDilutionRange : 6 µl/mg lysate , Application : WB , ApplicationDilutio
Anwendungsbeschreibung: Application Notes: Format: Whole IgG
Detection of human PVRL1/NECTIN1 (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784567) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.
Detection of human PVRL1/NECTIN1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 20% of IP loaded) from MCF-10A cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784567) used for IP at 6 µl/mg lysate.
Detection of human PVRL1/NECTIN1 by western blot. Samples: Whole cell lysate (50 µg) from MCF-10A, SNB-75, RT-4, MCF-7, and HaCaT cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PVRL1/NECTIN1 recombinant monoclonal antibody (orb1784567) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.