Rabbit anti-P-Selectin/CD62P Recombinant Monoclonal Antibody, Clone: [BLR268L], Unconjugated

Produktübersicht

• Artikelname: Rabbit anti-P-Selectin/CD62P Recombinant Monoclonal Antibody, Clone: [BLR268L], Unconjugated
• Artikelnummer: BYT-ORB1784574
• Hersteller Artikelnummer: orb1784574
• Alternativnummer: BYT-ORB1784574-100
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, ICC, IF, IHC, IP, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Residues 42-709 (ECD)
• Konjugation: Unconjugated
• Alternative Synonym: LECAM3, platelet activation dependent granule-external membrane protein, PADGEM, CD62P, leukocyte-endothelial cell adhesion molecule 3, P-selectin, GMP-140, CD62 antigen-like family member P, CD antigen CD62P, Grmp, granule membrane protein 140

Produktbeschreibung

Rabbit anti-P-Selectin/CD62P Recombinant Monoclonal Antibody

Produkteigenschaften

• Klonalität: Recombinant
• Konzentration: 1000 µg/ml
• Klon-Bezeichnung: [BLR268L]
• UniProt: Q01102
• Puffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Reinheit: Recombinant antibody was purified from cell culture supernatant
• Formulierung: Whole IgG
• Target-Kategorie: P-Selectin/CD62P

Anwendungsbeschreibung

• Application Verdünnung: Flow-Cyt Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10 6 cells. Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. Immunohistochemistr
• Anwendungsbeschreibung: Application Notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight

Bilder

Detection of mouse P-Selectin/CD62P (shaded) in b.END3 cells by flow cytometry. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Detection of human P-Selectin/CD62P (shaded) in Hel92.1.7 cells by flow cytometry. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Detection of mouse P-Selectin/CD62P by immunocytochemistry. Sample: FFPE section of bEnd.3 cells. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human P-Selectin/CD62P by immunocytochemistry. Sample: FFPE section of HEL 92.1.7 cells. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human P-Selectin/CD62P by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse P-Selectin/CD62P by immunohistochemistry. Sample: FFPE section of mouse ovary. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human P-Selectin/CD62P by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 20% of IP loaded) from HEL 92.1.7 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574) used for IP at 6 µl/mg lysate.

Detection of human P-Selectin/CD62P (orange) by immunofluorescence. Sample: FFPE section of metastatic lymph node. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574) used at 1:100. Secondary: Invitrogen Goat anti-rabbit IgG Alexa Fluor(TM) Plus 647 1:400. Counterstain: DAPI (blue).

Detection of human P-Selectin/CD62P by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Platelets, Jurkat, HEL 92.1.7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse P-Selectin/CD62P by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, bEnd.3 (10 µg) , CH27, Spleen, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.