CROP/LUC7L3 Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: CROP/LUC7L3 Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB1804684
• Hersteller Artikelnummer: orb1804684
• Alternativnummer: BYT-ORB1804684-100
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, FC, ICC, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: E.coli-derived human CROP/LUC7L3 recombinant protein (Position: D30-H211).
• Konjugation: Unconjugated
• Alternative Synonym: LUC7L3, CREAP1, CROP, O48, Luc7-like protein 3, Cisplatin resistance-associated-overexpressed protein, Luc7A, Okadaic acid-inducible phosphoprotein OA48-18, cAMP regulatory element-associated protein 1, CRE-associated protein 1, CREAP-1

Produktbeschreibung

Anti-CROP/LUC7L3 Antibody. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
• Molekulargewicht: 55 kDa
• UniProt: O95232
• Puffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Formulierung: Lyophilized
• Target-Kategorie: Luc7-like protein 3

Anwendungsbeschreibung

• Application Verdünnung: Western blot, 0.25-0.5 µg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 1-2 µg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human Flow Cytometry (Fixed), 1-3 µg/1x10 6 cells, Human ELISA, 0.1-0.5 µg/ml, -

Bilder

Flow Cytometry analysis of JK cells using anti-CROP/LUC7L3 antibody. Overlay histogram showing JK cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CROP/LUC7L3 Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

WB analysis of CROP/LUC7L3 using anti-CROP/LUC7L3 antibody.Lane

IF analysis of CROP/LUC7L3 using anti-CROP/LUC7L3 antibody and anti-Beta Tubulin antibody. CROP/LUC7L3 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-CROP/LUC7L3 Antibody and mouse anti-Beta Tubulin antibody overnight at 4C. DyLight488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IHC analysis of CROP/LUC7L3 using anti-CROP/LUC7L3 antibody. CROP/LUC7L3 was detected in a paraffin-embedded section of human highly differentiated adenocarcinoma of the colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-CROP/LUC7L3 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of CROP/LUC7L3 using anti-CROP/LUC7L3 antibody. CROP/LUC7L3 was detected in a paraffin-embedded section of human large B-cell lymphoma of the colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-CROP/LUC7L3 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of CROP/LUC7L3 using anti-CROP/LUC7L3 antibody. CROP/LUC7L3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-CROP/LUC7L3 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of CROP/LUC7L3 using anti-CROP/LUC7L3 antibody. CROP/LUC7L3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-CROP/LUC7L3 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of CROP/LUC7L3 using anti-CROP/LUC7L3 antibody. CROP/LUC7L3 was detected