General 1, 25-Dihydroxyvitamin D3 (DHVD3) ELISA Kit
Detektionsbereich:
156.25-10000pg/mL
Sensitivitaet:
49.5 pg/mL
Proben:
serum, plasma and other biological fluids
Target-Kategorie:
DHVD3
Anwendungsbeschreibung:
Application Notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,25-Dihydroxyvitamin D3(DHVD3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,25-Dihydroxyvitamin D3(DHVD3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of 1,25-Dihydroxyvitamin D3(DHVD3) in the samples is then determined by comparing the OD of the samples to the standard curve
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