General Cyclic Guanosine Monophosphate (cGMP) ELISA Kit

Artikelnummer: BYT-ORB1806554
Artikelname: General Cyclic Guanosine Monophosphate (cGMP) ELISA Kit
Artikelnummer: BYT-ORB1806554
Hersteller Artikelnummer: orb1806554
Alternativnummer: BYT-ORB1806554-48,BYT-ORB1806554-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Spezies Reaktivität: All
Alternative Synonym: c-GMP, 3,5-Cyclic GMP, Cyclic 3,5-GMP, Guanosine 3,5-Cyclic Phosphate
General Cyclic Guanosine Monophosphate (cGMP) ELISA Kit
Detektionsbereich: 156.25-10000pg/mL
Sensitivitaet: 45.2 pg/mL
Proben: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Target-Kategorie: cGMP
Anwendungsbeschreibung: Application Notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cyclic Guanosine Monophosphate(cGMP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cyclic Guanosine Monophosphate(cGMP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Cyclic Guanosine Monophosphate(cGMP) in the samples is then determined by comparing the OD of the samples to the standard curve
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