E.coli-derived human Cofilin recombinant protein (Position: A2-L166). Human Cofilin shares 99% amino acid (aa) sequences identity with both mouse and rat Cofilin.
Application Notes: Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat, Monkey Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1µg/ml, Human Immunocytochemistry/Immunofluorescence, 2µg/ml, Human Flow Cytometry (Fixed), 1-3µg/1x106 cells, Human ELISA, 0.1-0.5µg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Flow Cytometry analysis of U20S cells using anti-Cofilin antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of Cofilin using anti-Cofilin antibody.Lane 1:human HeLa cell,2:monkey COS-7 cell,3:human HEK293 cell,4:human U-87MG cell,5:human HepG2 cell,6:human PC-3 cell,7:human THP-1 cell,8:human A549 cell.