EIF4A3 Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: EIF4A3 Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB183348
• Hersteller Artikelnummer: orb183348
• Alternativnummer: BYT-ORB183348-100,BYT-ORB183348-200,BYT-ORB183348-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human eIF4A3 (351-411/411aa)
• Konjugation: Unconjugated
• Alternative Synonym: ATP-dependent RNA helicase DDX48, ATP-dependent RNA helicase eIF4A-3, DDX48, DEAD box protein 48, eIF-4A-III, eIF4A-III, EIF4A3, eIF4AIII, Eukaryotic initiation factor 4A-III, Eukaryotic initiation factor 4A-like NUK-34, EUKARYOTIC TRANSLATION INITIATION FACTOR 4A ISOFORM 3, hNMP 265, IF4A3_HUMAN, NMP 265, NMP265, NUCLEAR MATRIX PROTEIN 265, NUK34.

Produktbeschreibung

EIF4A3 Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 47 kDa
• UniProt: P38919
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: EIF4A3

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test

Bilder

Immunohistochemical staining of mouse embryo tissue using eIF4A3 antibody.

Western blot analysis of extracts from placenta using elf4A3 antibody.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-eIF4A3 antibody (orb183348), dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (eIF4A3) polyclonal Antibody, Unconjugated (orb183348) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (eIF4A3) Polyclonal Antibody, Unconjugated (orb183348) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse embryos tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (eIF4A3) Polyclonal Antibody, Unconjugated (orb183348) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (eIF4A3) Polyclonal Antibody, Unconjugated (orb183348) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Rat Testis tissue lysates, Lane 3: Human HepG2 cell lysates, Lane 4: Human HeLa cell lysates, Lane 5: Human MCF-7 cell lysates, Primary: Anti-eIF4A3 (orb183348) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kDa, Observed band size: 47 kDa.

Tissue/Cell: mouse embryo tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-eIF4A3 Polyclonal Antibody, Unconjugated (orb183348) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.