Phospho-Ku70 / XRCC6 (Ser5) Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: Phospho-Ku70 / XRCC6 (Ser5) Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB184612
• Hersteller Artikelnummer: orb184612
• Alternativnummer: BYT-ORB184612-100,BYT-ORB184612-200,BYT-ORB184612-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Mouse
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Ku70 around the phosphorylation site of Ser5 WE(p-S)YY
• Konjugation: Unconjugated
• Alternative Synonym: XRCC6_HUMAN, X-ray repair cross-complementing protein 6, G22P1, EC:3.6.4.-, EC:4.2.99.-, 5-deoxyribose-5-phosphate lyase Ku70(5-dRP lyase Ku70), 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box-binding factor 75 kDa subunit(CTC75, CTCBF), ML8, KU70, KU-70, TLAA,

Produktbeschreibung

Phospho-Ku70 / XRCC6 (Ser5) Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 70 kDa
• UniProt: P12956
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: XRCC6

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Anwendungsbeschreibung: Modification: Phosphorylated

Bilder

Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-Ku70 (Ser5)) Polyclonal Antibody, Unconjugated (orb184612) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Heart tissue lysates, Lane 2: Human HL-60 cell lysates, Primary: Anti-phospho-Ku70 (Ser5) (orb184612) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 70 kDa, Observed band size: 70 kDa.