Keratin 36 Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: Keratin 36 Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB184679
• Hersteller Artikelnummer: orb184679
• Alternativnummer: BYT-ORB184679-100,BYT-ORB184679-200,BYT-ORB184679-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human
• Immunogen: KLH conjugated synthetic peptide derived from human Keratin 36 (301-400/467aa)
• Konjugation: Unconjugated
• Alternative Synonym: HA6, Hair keratin, Hair keratin, type I Ha6, HHA6, HKA6, K36, Keratin, Keratin hair acidic 6, Keratin type I cuticular Ha6, keratin, hair, acidic, 6, Keratin, hard, type I, Keratin, hard, type I, 6, keratin, type I cuticular Ha6, Keratin-36, Krt36, KRT36_HUMAN, KRTHA6, type I cuticular Ha6, type I Ha6.

Produktbeschreibung

Keratin 36 Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 52 kDa
• UniProt: O76013
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: KRT36

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Bilder

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Keratin 36) Polyclonal Antibody, Unconjugated (orb184679) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: HL-60 Cell (Human) Lysate at 30 ug, Primary: Anti-Keratin (orb184679) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 52 kD, Observed band size: 60 kD.