L2HGDH Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB184839
Artikelname: L2HGDH Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB184839
Hersteller Artikelnummer: orb184839
Alternativnummer: BYT-ORB184839-100,BYT-ORB184839-200,BYT-ORB184839-50
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Mouse
Immunogen: KLH conjugated synthetic peptide derived from human L2HGDH (201-300/463aa)
Konjugation: Unconjugated
Alternative Synonym: 2 hydroxyglutarate dehydrogenase, alpha hydroxyglutarate oxidoreductase, alpha ketoglutarate reductase, C14orf160, Duranin, FLJ12618, L alpha hydroxyglutarate dehydrogenase, L-2-hydroxyglutarate dehydrogenase, L-2-hydroxyglutarate dehydrogenase, mitochondrial, L2HDH_HUMAN, l2hgdh, mitochondrial.
L2HGDH Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 45 kDa
UniProt: Q9H9P8
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: L2HGDH
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (L2HGDH) Polyclonal Antibody, Unconjugated (orb184839) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Pancreas (Mouse) Lysate at 40 ug, Primary: Anti-L2HGDH (orb184839) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 48 kD.