Phospho-LIM Kinase 2 (Thr505) Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB184929
Artikelname: Phospho-LIM Kinase 2 (Thr505) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB184929
Hersteller Artikelnummer: orb184929
Alternativnummer: BYT-ORB184929-100,BYT-ORB184929-200,BYT-ORB184929-50
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P
Spezies Reaktivität: Rat
Immunogen: KLH conjugated synthesised phosphopeptide derived from human LIM Kinase 1 around the phosphorylation site of Thr505 RY(p-T)VV
Konjugation: Unconjugated
Alternative Synonym: LIM Kinase 1(phospho T505), p-LIM Kinase 1(phospho T505), LIM domain kinase 2, LIMK 2, LIMK-2, LIMK2, LIMK2_HUMAN.
Phospho-LIM Kinase 2 (Thr505) Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 70 kDa
UniProt: P53671
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: LIMK2
Application Verdünnung: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Anwendungsbeschreibung: Modification: Phosphorylated
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-LIM Kinase 2 (Thr505)) Polyclonal Antibody, Unconjugated (orb184929) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-LIM Kinase 2 (Thr505)) Polyclonal Antibody, Unconjugated (orb184929) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.