Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MAPKAP1/SIN1) Polyclonal Antibody, Unconjugated (orb185163) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-MAPKAP1/SIN1 (orb185163) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 30/50/65 kD, Observed band size: 50 kD.
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