Phospho-NFKB p65 (Ser281) Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: Phospho-NFKB p65 (Ser281) Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB185656
• Hersteller Artikelnummer: orb185656
• Alternativnummer: BYT-ORB185656-100,BYT-ORB185656-200,BYT-ORB185656-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human NFKB p65 around the phosphorylation site of Ser281 EL(p-S)EP
• Konjugation: Unconjugated
• Alternative Synonym: TF65_HUMAN, Transcription factor p65, RELA, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, NFKB3, RELA proto-oncogene, NF-kB subunit, p65, CMCU, NFKB3, AIF3BL3, NF-kB p65/RelA, NFkappaB-p65, NFkappaB p65, NF kappaB-p65, NFkappaBp65,

Produktbeschreibung

Phospho-NFKB p65 (Ser281) Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 61 kDa
• UniProt: Q04206
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: RELA

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, Flow-Cyt=1µg/Test
• Anwendungsbeschreibung: Modification: Phosphorylated

Bilder

Flow cytometric analysis of Jurkat cells (blue) using NFkB p65 (phospho-Ser281) antibody.

Flow cytometric analysis of Green line cell using NFkB p65 (phospho-Ser281) antibody.

Blank control (blue): Jurkat cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-phospho-NFKB p65 (Ser281) antibody antibody (orb185656), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-phospho-NFKB p65 (Ser281) antibody (orb185656), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-phospho-NFKB p65 (Ser281) antibody (orb185656), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-phospho-NFKB p65 (Ser281) antibody (orb185656), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Mouse thymus. Primary Antibody (green line): Rabbit Anti-phospho-NFKB p65 (Ser281) antibody (orb185656), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Overlay histogram showing HL 60 cells stained with orb185656 (Green line). The cells were fixed with 90% methanol (5 min) and then permeabilized with 0.01M PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (orb185656, 1 µg/1x10 6 cells) for 30 min at 22C. The secondary antibody used was fluorescein isothiocyanate goat anti-rabbit IgG (H+L) (Brillant blue line) at 1/200 dilution for 30 min at 22C. Isotype control antibody was rabbit IgG (polyclonal, Orange line) (1 µg/1x10 6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of 20000 events were colle