Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application Verdünnung:
WB - 1:1000
Western blot analysis of USP22 Antibody (N-term) in Jurkat cell line lysates (35 ug/lane).USP22 (arrow) was detected using the purified Pab.
All lanes: Anti-USP22 Antibody (S71) at 1:1000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: NCI-H1299 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 60 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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