Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application Verdünnung:
WB - 1:2000
Confocal immunofluorescent analysis of TGFBR2 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
All lanes: Anti-TGFBR2 Antibody (N-term) at 1:2000 dilution. Lane 1: NCCIT whole cell lysates. Lane 2: mouse lung lysates. Lane 3: SW620 whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-TGFBR2 Antibody (N-term) at 1:2000 dilution + mouse lung lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-TGFBR2 Antibody (N-term) at 1:2000 dilution. Lane 1: human testis lysate. Lane 2: mouse lung lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-TGFBR2 Antibody (N-term) at 1:2000 dilution + mouse lung lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
TGFBR2 Antibody (N-term) western blot analysis in mouse lung tissue lysates (35 ug/lane). This demonstrates the TGFBR2 antibody detected the TGFBR2 protein (arrow).
Anti-TGFBR2 Antibody (N-term) at 1:2000 dilution + mouse lung lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 67 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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