CD130 Antibody (C-term), Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: CD130 Antibody (C-term), Unconjugated, Rabbit, Polyclonal
• Artikelnummer: BYT-ORB1938446
• Hersteller Artikelnummer: orb1938446
• Alternativnummer: BYT-ORB1938446-100,BYT-ORB1938446-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IHC-P, WB
• Spezies Reaktivität: Human
• Konjugation: Unconjugated

Produktbeschreibung

CD130 Antibody (C-term)

Produkteigenschaften

• Klonalität: Polyclonal
• Molekulargewicht: 103537 Da
• UniProt: P40189
• Formulierung: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Anwendungsbeschreibung

• Application Verdünnung: IHC-P - 1:100-500, WB - 1:2000

Bilder

All lanes: Anti-CD130 Antibody (C-term) at 1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 104 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CD130 Antibody (C-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: MDA-MB-231 whole cell lysate. Lane 3: SW480 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 104 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CD130 Antibody (C-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: MDA-MB-231 whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: SW480 whole cell lysate. Lane 5: A549 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 104 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

CD130 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35 ug/lane). This demonstrates the CD130 antibody detected the CD130 protein (arrow).

Anti-CD130 Antibody (C-term) at 1:2000 dilution + SW480 whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 104 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CD130 Antibody (C-term) at 1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: HT-1080 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size:104kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining CD130 in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37C. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.