GAPDH Antibody, IgG1, Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: GAPDH Antibody, IgG1, Unconjugated, Mouse, Monoclonal
• Artikelnummer: BYT-ORB1939451
• Hersteller Artikelnummer: orb1939451
• Alternativnummer: BYT-ORB1939451-100,BYT-ORB1939451-50
• Hersteller: Biorbyt
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: IF, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Konjugation: Unconjugated

Produktbeschreibung

GAPDH Antibody

Produkteigenschaften

• Klonalität: Monoclonal
• Molekulargewicht: 36053 Da
• Isotyp: IgG1
• UniProt: P04406
• Formulierung: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Anwendungsbeschreibung

• Application Verdünnung: WB - 1:1000, IHC-P - 1:100-500, IF - 1:25

Bilder

All lanes: Anti-GAPDH Antibody at 1:1000 dilution + C6 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 36KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of Hela cells stained with XAF1 GAPDH Antibody. diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

All lanes: Anti-GAPDH Antibody at 1:1000 dilution. Lane 1: A431 whole cell lysates. Lane 2: C6 whole cell lysates. Lane 3: Hela whole cell lysates. Lane 4: HUVEC whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-GAPDH Antibody at 1:8000 dilution. Lane 1: Hela whole cell lysates. Lane 2: A549 whole cell lysates. Lane 3: COS-7 whole cell lysates. Lane 4: mouse brain lysates. Lane 5: C6 whole cell lysates. Lane 6: NIH/3T3 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-GAPDH Monoclonal Antibody in CEM cell line lysates (35 µg/lane). GAPDH (arrow) was detected using the purified Mab.

Immunohistochemical analysis of paraffin-embedded H.kidney section using GAPDH Antibody. diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H.stomach section using GAPDH Antibody. diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.