SARS-CoV-2 Epsilon 427 Variant Recombinant Spike RBD His Tag
Konzentration:
0.5 mg/ml
Klon-Bezeichnung:
[COV2-Epsilon427]
Puffer:
1X PBS, 0.025% NaN3
Application Verdünnung:
Control antigen for SARS-CoV-2 (COVID-19) diagnostic assays. Contains C-terminal His tag used for purification.
Concentration-response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4?C overnight. The wells were washed with PBS and blocked with 200 µL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 µg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 µL of serially diluted ACE2-Fc at 37?C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 µL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc? fragment specific (1:5, 000 in 1% BSA/PBS) at 37?C for 1 hour. The wells were washed with PBS and the wells were developed with 100 µL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate at RT for 5 min. The reaction was stopped with 100 µL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader.
SARS-CoV-2 spike protein variant purity by SDS-PAGE. Separation of SARS-CoV-2 spike recombinant protein (8xHis-Tag, 2 ug) reduced (R) and non-reduced (NR) condition followed by Coommassie blue staining shows >90% purity.
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