Rat Secondary Lymphoid Tissue Chemokine (SLC) ELISA Kit

Artikelnummer: BYT-ORB1949989
Artikelname: Rat Secondary Lymphoid Tissue Chemokine (SLC) ELISA Kit
Artikelnummer: BYT-ORB1949989
Hersteller Artikelnummer: orb1949989
Alternativnummer: BYT-ORB1949989-48, BYT-ORB1949989-96
Hersteller: Biorbyt
Kategorie: Kits/Assays
Spezies Reaktivität: Rat
Alternative Synonym: CCL21, 6Ckine, CKb9, ECL, SCYA21, TCA4
Rat Secondary Lymphoid Tissue Chemokine (SLC) ELISA Kit
Detektionsbereich: 62.5-4000pg/mL
Sensitivitaet: 37.5 pg/mL
UniProt: A0A8I5ZU27
Proben: serum, plasma, Tissue homogenate and Other biological samples
Target-Kategorie: SLC
Anwendungsbeschreibung: Application Notes: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SLC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SLC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SLC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Rat SLC. You can calculate the concentration of Rat SLC in the samples by comparing the OD of the samples to the standard curve