E.coli-derived mouse E-cadherin/Cdh1 recombinant protein (Position: Q23-Q708). Mouse Cdh1 shares 77.9% and 90.7% amino acid (aa) sequence identity with human and rat Cdh1, respectively.
Konjugation:
Unconjugated
Anti-E-cadherin/Cdh1 Antibody. Tested in WB, IHC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Mouse, Rat.
Klonalität:
Polyclonal
Konzentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Application Notes: Western blot, 0.25-0.5 µg/ml, Mouse Immunohistochemistry, 2-5 µg/ml, Mouse, Rat Immunofluorescence, 5 µg/ml, Human Flow Cytometry (Fixed), 1-3 µg /1x106 cells, Mouse ELISA, 0.1-0.5 µg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml
Flow Cytometry analysis of NIH/3T3 cells using anti-E-Cadherin/Cdh1 antibody. Overlay histogram showing NIH/3T3 cells (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-E-Cadherin/Cdh1 Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
IF analysis of E-Cadherin/Cdh1 using anti-E-Cadherin/Cdh1 antibody. E-Cadherin/Cdh1 was detected in a paraffin-embedded section of mouse stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-E-Cadherin/Cdh1 Antibody overnight at 4C. DyLight594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IF analysis of E-Cadherin/Cdh1 using anti-E-Cadherin/Cdh1 antibody. E-Cadherin/Cdh1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-E-Cadherin/Cdh1 Antibody overnight at 4C. DyLight594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IF analysis of E-Cadherin/Cdh1 using anti-E-Cadherin/Cdh1 antibody. E-Cadherin/Cdh1 was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-E-Cadherin/Cdh1 Antibody overnight at 4C. DyLight594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of E-Cadherin/Cdh1 using anti-E-Cadherin/Cdh1 antibody. E-Cadherin/Cdh1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-E-Cadherin/Cdh1 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of E-Cadherin/Cdh1 using anti-E-Cadherin/Cdh1 antibody. E-Cadherin/Cdh1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-E-Cadherin/Cdh1 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of E-Cadherin/Cdh1 using anti-E-Cadherin/Cdh1 antibody. E-Cadherin/Cdh1 was detected in a paraffin-embedded sect
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