anti NOVA1 antibody, anti neuro-oncological ventral antigen 1 antibody, anti Nova-1 antibody, anti paraneoplastic Ri antigen antibody, anti ventral neuron-specific protein 1 antibody
Goat polyclonal antibody to NOVA1
Klonalität:
Polyclonal
Molekulargewicht:
51.7, 19.5
Puffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Sequenz:
REMPQNVAKTEPVS
Target-Kategorie:
NOVA1
Application Verdünnung:
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx. 50-55kDa band observed in Human Breast Cancer lysates, and in preliminary testing of Human Kidney lysate (calculated MW of 51.7kDa according to NP_002506.2). Recommended conce
orb19796 (0.01ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing NOVA1 (RC210407) and probed with orb19796 (mock transfection in first lane), tested by Origene.
orb19796 (2.5ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).
Primary incubation 1 hour at room temperature. Image A: Human Breast Cancer lysate at primary Ab concentration 0.03 µg/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.
Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
5 µg/ml staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH6, AP-staining.
5 µg/ml staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH6, AP-staining.
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