Human Protein Inhibitor of Activated STAT4 (PIAS4) ELISA Kit
Artikelnummer:
BYT-ORB1994902
Hersteller Artikelnummer:
orb1994902
Alternativnummer:
BYT-ORB1994902-48, BYT-ORB1994902-96
Hersteller:
Biorbyt
Kategorie:
Kits/Assays
Spezies Reaktivität:
Human
Alternative Synonym:
PIASY, Piasg, ZMIZ6, PIAS-gamma
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIAS4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIAS4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIAS4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human PIAS4 in the samples is then determined by comparing the OD of the samples to the standard curve.
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Anwendungsbeschreibung:
Application Notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIAS4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIAS4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIAS4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human PIAS4 in the samples is then determined by comparing the OD of the samples to the standard curve
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