RNF113A Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: RNF113A Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB2068
• Hersteller Artikelnummer: orb2068
• Alternativnummer: BYT-ORB2068-50,BYT-ORB2068-100,BYT-ORB2068-200
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human RNF113A (51-150/343aa)
• Konjugation: Unconjugated
• Alternative Synonym: Cwc24, RNF113, TTD5, ZNF183, R113A_HUMAN, RNF113A, Cwc24 homolog, RING finger protein 113A, Zinc finger protein 183, 2.3.2.27,

Produktbeschreibung

RNF113A Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 39 kDa
• UniProt: O15541
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: RNF113A

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

Bilder

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNF113A) Polyclonal Antibody, Unconjugated (orb2068) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNF113A) Polyclonal Antibody, Unconjugated (orb2068) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNF113A) Polyclonal Antibody, Unconjugated (orb2068) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (RNF113A) Polyclonal Antibody, Unconjugated (orb2068) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-RNF113A (orb2068) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39 kD, Observed band size: 39 kD.

IHC-P analysis of rat colon using RNF113A Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of mouse brain using RNF113A Polyclonal Antibody at 1:200 dilution.