Goat Mouse IgE (Fc specific), conjugated with Biotin Antibody, Polyclonal

Produktübersicht

• Artikelname: Goat Mouse IgE (Fc specific), conjugated with Biotin Antibody, Polyclonal
• Artikelnummer: BYT-ORB21603
• Hersteller Artikelnummer: orb21603
• Alternativnummer: BYT-ORB21603-1
• Hersteller: Biorbyt
• Wirt: Goat
• Kategorie: Antikörper
• Spezies Reaktivität: Mouse
• Konjugation: Biotin

Produktbeschreibung

Biotin-conjugated IgG fraction of polyclonal goat antiserum to mouse IgE, Fc specific. In immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the users choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1: 250, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:2,000.

Produkteigenschaften

• Klonalität: Polyclonal
• Formulierung: Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.