Goat Dog IgG (heavy and light chains), conjugated with Biotin Antibody, Polyclonal
Artikelnummer:
BYT-ORB22031
Hersteller Artikelnummer:
orb22031
Alternativnummer:
BYT-ORB22031-1
Hersteller:
Biorbyt
Wirt:
Goat
Kategorie:
Antikörper
Spezies Reaktivität:
Canine
Konjugation:
Biotin
Biotin-conjugated IgG fraction of polyclonal goat antiserum to Dog IgG, heavy and light chains. In immunocytochemical and immunohistochemical staining to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of dog origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the users choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions fro histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:10,000.
Klonalität:
Polyclonal
Formulierung:
Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
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