E.coli-derived human Peroxiredoxin 5 recombinant protein (Position: E66-D198). Human Peroxiredoxin 5 shares 91% amino acid (aa) sequence identity with both mouse and rat Peroxiredoxin 5.
Application Notes: Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2µg/ml, Human Flow Cytometry (Fixed), 1-3µg/1x106 cells, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
IHC(P) analysis of Mouse Intestine Tissue using Anti-Peroxiredoxin 5 Picoband antibody.
Anti-Peroxiredoxin 5 Picoband antibody, IHC(P): Human Lung Cancer Tissue.
IHC(P) analysis of Rat Intestine Tissue using Anti-Peroxiredoxin 5 Picoband antibody.
Anti-Peroxiredoxin 5 Picoband antibody, IHC(P): Rat Intestine Tissue.
Anti-Peroxiredoxin 5 Picoband antibody, Western blotting. All lanes: Anti Peroxiredoxin 5 at 0.5 ug/ml. Lane 1: A549 Whole Cell Lysate at 40 ug. Lane 2: Rat Brain Tissue Lysate at 50 ug. Lane 3: Mouse Brain Tissue Lysate at 50 ug. Predicted bind size: 22 KD. Observed bind size: 22 KD.
Flow Cytometry analysis of A549 cells using anti-Peroxiredoxin 5 antibody. Overlay histogram showing A549 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Peroxiredoxin 5 Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of Peroxiredoxin 5 using anti-Peroxiredoxin 5 antibody. Peroxiredoxin 5 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 µg/mL rabbit anti-Peroxiredoxin 5 Antibody overnight at 4C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of Peroxiredoxin 5 using anti-Peroxiredoxin 5 antibody. Peroxiredoxin 5 was detected in immunocytochemical section of SMMC Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 µg/ml rabbit anti-Peroxiredoxin 5 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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